教學

開授科目：

專長與研究

研究專長：
　　前驅抗體藥物、電腦&抗體基因工程(人類化抗體)、蛋白質/抗體標靶藥物、功能性分子造影、腫瘤免疫學
休閒興趣：
　　釣魚/蝦、看漫畫、羽毛球、下棋/麻將、研究/看papers誘創思

研究領域：

　　實驗室目前主要研究領域: 1. 萬能抗體鎖研究 (鎖盡天下名刀) 2.電腦結構輔助抗體人類化研究 (電腦做抗體) 3. 人類B 細胞不死化平台(全人化抗體藥開發) 4. 以抗聚乙二醇雙功能抗體非共價鍵修飾聚乙二醇-奈米分子以進行腫瘤標的治療5. Anti-PEG Ab生醫應用 (產業化應用) 6.功能性分子造影7.其他共>100研究主題

　　1. 萬能抗體鎖研究 (鎖盡天下名刀): 讓抗體藥更具選擇性

　　抗體藥物已為現今臨床主流(2013年產值540億美金)藥物，但其所辨認的抗原亦於正常組織表現，長期使用常易產生副作用。因此如何再增加抗體選擇性有其必要性，我們的策略是利用抗體自身之Hinge domain結構作為抗體鎖(閉鎖器)，以蛋白酶受質胜肽連結抗體和此閉鎖器，形成具屏蔽抗體結合能力之閉鎖器-抗體，唯有在蛋白酶過度表現的疾病區，閉鎖器才能被移除，回復抗體功能，增加抗體對疾病區域之選擇性，並降低全身性副作用，提供病人更好的生活品質。未來抗體鎖每鎖1抗體藥就可產生1技轉，可多次技轉給全球各大藥廠，預計其專利具有100億美元以上的商機。(目前進行中疾病抗體:類風濕性關節炎(Humira、Remicade)、骨質疏鬆症(Prolia)、動脈硬化 (anti-VCAM)、牛皮癬(Stelara、Raptiva)及癌症(Herceptin、Yervoy、Panitumumab、Tremelimumab)

圖一：可提升抗體藥物選擇性的萬能抗體鎖:抗體鎖可鎖盡天下所有名刀(抗體)的結合能力，唯有在疾病區，抗體鎖才能被移除，回復抗體功能，增加抗體對疾病治療之選擇性，並降低全身性副作用。假設，每一個抗體都是一把名刀，在我們的體內殺遍抗原，以治療疾病，但不是所有的抗原都是壞人，不能全殺無赦，因此就需要" 刀鎖"的存在。刀鎖就像刀鞘，使名刀(抗體)在正常組織藏匿刀鞘裡不露鋒芒，唯有到達疾病區域時才會出鞘，砍殺壞抗原，降低名刀全身性鋒芒畢露的致命缺點(副作用)。本刀鎖可鎖盡天下名刀，讓其靈性(選擇性)升級，未來適用於提升全球百大藥廠的名刀，增加其疾病治療時的靈性，因此商機無限!

　　2. 電腦結構輔助抗體人類化研究 (電腦做抗體)：抗體人類化與最佳化
　　抗體是人體最天然的藥物，所謂"天然的最好"抗體新藥已成為目前國際醫藥市場上最重要的藥物主流，然而30年來全世界累積抗體的研究能量，95%抗體仍以老鼠抗體為主，若能夠將其人類化甚至全人類化，將可以有效加速這些抗體藥物進入臨床應用。傳統進行抗體人類化方式，抗體往往會大大降低活性甚至失去功能，若能配合電腦結構模擬輔助抗體(含全人類化)之基因工程技術，將可依不同鼠源抗體，量身打造接近原抗體親和力之人類化甚至全人類化抗體，徹底改革傳統抗體人類化"有功能就好"的傳統觀念，減低抗體藥物開發的時程與成本，讓更多的抗體藥物可以快速進到臨床應用。

圖二：電腦結構輔助抗體人類化

　　3. 人類B 細胞不死化平台(全人化抗體藥開發)
　　過去研究發現，癌症組織內會累積腫瘤浸潤B細胞(Tumor-infiltrating B lymphocyte; TIL-B)，而這些B細胞所分泌之抗體，對癌症組織具有專一性。將B細胞與細胞膜上表現CD40 ligand (CD40L)的細胞共同培養及送入Bcl-2-like protein 1 (BCL-2L-1)及B-cell lymphoma 6 protein (BCL-6)兩種基因，使B細胞達到不死化(immortalized)的效果，建立各種病患(癌症、白斑、病毒或其他感染者)的不死化B細胞抗體庫，以利診斷抗體及全人化治療性抗體之篩選，一旦篩選到中和性抗體藥物就是全人化抗體，不需人類化可大大加速抗體進任臨床之進程。

圖三：治療性標靶抗體篩選平台: 建立各種病患(癌症、自體免疫疾病/白斑...、病毒或其他感染者)的不死化B細胞抗體庫，以利診斷抗體及全人化治療性抗體之篩選，一旦篩選到中和性抗體藥物就是全人化抗體，不需人類化工程，即可大大加速抗體進任臨床之進程。

　　4. 以人類化雙功能抗體單一步驟改變聚乙二醇-奈米分子專一性以進行腫瘤標靶治療(One step mixing of mPEGylated nanoparticles with humanized bispecific antibodies for cancer targeted therapy)

期刊論文

選擇性著作（近五年內選擇性著作10篇）

1. Kuo-Hsiang Chuang, Hsin-Ell Wang, Ta-Chun Cheng, Shey-CherngTzou, Chiu-Min Cheng, Chien-Han Kao, Chih-Hung Chuang3, Wei-Lung Tseng, Wen-Chun Hung, Ming-Hong Tai, Tien-Kuei Chang, Bing-Mae Chen, Steve R. Roffler and Tian-Lu Cheng*. Development of anUniversalAnti-Polyethylene Glycol Reporter Gene for Noninvasive Imaging of PEGylated Probes. Journal of Nuclear Medicine,2010, 51:933-941. 2012 IF: 5.774 (6/120, 5%) in RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
2. Kuo-Hsiang Chuang, Shey-CherngTzou, Ya-Chen Chang,Chien-Han Kao, Kuang-Wen, Liao, Ta-Chun Cheng, Bo-Jyun Huang, Chang-Jer Wu, Wei-Lung Tseng, JentaieShiea, Pei-Yu Chu, Steve Roffler, Tian-Lu Cheng* Measurement of Free Polyethylene Glycolby a Novel Cell-Based Anti-Polyethylene Glycol ELISA. Anal Chem. 2010 Mar 15;82(6):2355-62. 2012 IF: 5.695 (3/75, 4%) in CHEMISTRY, ANALYTICAL
3. Cheng TL, Chuang KH, Chen BM, Roffler SR. Analytical Measurement of PEGylated Molecules. Bioconjug Chem. 2012 Feb 2.23, 881−8992012 IF=4.580 (8/55=14.5%) in CHEMISTRY, ORGANIC
4.  Kuo-Hsiang Chuang, Hsin-Ell Wang, Fang-Ming Chen, Shey-CherngTzou, Chiu-Min Cheng, Ya-Chen Chang, Wei-Lung Tseng, JentaieShiea, Shiu-Ru Lin, Jaw-Yuan Wang, Bing-Mae Chen, Steve R. Roffler andTian-Lu Cheng*. Endocytosis of PEGylated agents enhances cancer imaging and anti-cancer efficacy. Molecular cancer therapeutics, 2010, 9(6);1903-1912. 2012 IF=5.599 (29/197=14.72%) in ONCOLOGY
5. CH Chuang, KH Chuang, HE Wang, SR Roffler, SC Tzou, TC Cheng, CH Kao, SY Wu, WL Tseng, J Shiea, JM Wang* and TL Cheng*. In vivo PET Imaging of protease activity by hydrophilic-to-hydrophobic conversion of a protease substrate.Clin Cancer Res. 2012 Jan 1;18(1):238-247.2012 IF=7.837 (12/196=6.1%) in ONCOLOGY
6. Cheng TC, Chuang KH, Chen M, Wang HE, Tzou SC, Su YC, Chuang CH, Kao CH, Chen BM, Chang LS, Roffler SR, Cheng TL*. Sensitivity of PEGylated interferon detection by anti-polyethylene glycol (PEG) antibodies depends on PEG length.Bioconjug Chem. 2013 Jul 9. [Epub ahead of print]2012 IF=4.580(8/55=14.5%) in CHEMISTRY, ORGANIC
7. Su YC, Chen BM, Chuang KH, Tian-Lu Cheng*,Roffler SR. Sensitive Quantification of PEGylated Compounds by Second-Generation Anti-Poly(ethylene glycol) Monoclonal Antibodies.Bioconjug Chem. 2010 Jul 21;21(7):1264-70.2012IF=4.580(8/55=14.5%) in CHEMISTRY, ORGANIC
8. Ta-Chun Cheng, Steve R. Roffler, Shey-CherngTzou, Kuo-Hsiang Chuang, Yu-Cheng Su, Chih-Hung Chuang, Chien-Han Kao,Chien-Shu Chen,I-Hong Harn, Kuan-Yi Liu,Tian-Lu Cheng*and Yu-Ling Leu*.An Activity-Based Infrared Glucuronide Trapping Probe for Imaging b-Glucuronidase Expression in Deep Tissues.J. Am. Chem. Soc. 2012, 134, 3103−3110.2012 IF=10.677 (11/152=7.2%) in CHEMISTRY, MULTIDISCIPLINARY
9. Shey-CherngTzou, Steve Roffler, Kuo-Hsiang Chuang, Hsin-Pei Yeh, Chien-Han Kao, Yu-Cheng Su, Chiu-Min Cheng, Wei-Lung Tseng, JentaieShiea, Kai-Wen Cheng, Bing-Mae Chen, Tian-Lu Cheng*and Hsin-Ell Wang. Micro-PET imaging of b-glucuronidase activity by the hydrophobic conversion of a glucuronide probe. Radiology, 2009, Sep;252(3):754-62. 2012 IF=6.339 (2/120=1.67%) in RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
10. Tian-Lu Cheng and Steve Roffler. Membrane-tethered proteins for basic research, imaging and therapy. Medicinal Research Reviews, 2008, Nov;28(6):885-928. 2012 IF= 9.583 (5/261=1.9 %) in PHARMACOLOGY & PHARMACY

2008~2014 Publications
2014 publications

1. Cheng CM, Tzou SC, Zhuang YH, Huang CC, Kao CH, Liao KW, Cheng TC, Chuang CH, Hsieh YC, Tai MH, Cheng TL*. Functional production of a soluble and secreted single-chain antibody by a bacterial secretion system. PLoS One. 2014 May 13;9(5):e97367
2. Chen YJ, Lin HC, Chen KC, Lin SR, Cheng TL, Chang LS. Taiwan cobra phospholipase A₂ suppresses ERK-mediated ADAM17 maturation, thus reducing secreted TNF-α production in human leukemia U937 cells. Toxicon. 2014 May 27;86C:79-88.
3. Su YC, Al-Qaisi TS, Tung HY, Cheng TL, Chuang KH, Chen BM, Roffler SR. Mimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-PEG antibodies. MAbs. 2014 May 14;6(4).
4. Chuang CH, Wang WJ, Li CF, Ko CY, Chou YH, Chuu CP, Cheng TL*, Wang JM*. The combination of the prodrugs perforin-CEBPD and perforin-granzyme B efficiently enhances the activation of caspase signaling and kills prostate cancer. Cell Death Dis. 2014 May 8;5:e1220
5. Chen YJ, Wu SC, Chen CY, Tzou SC, Cheng TL, Huang YF, Yuan SS, Wang YM. Peptide-based MRI contrast agent and near-infrared fluorescent probe for intratumoral legumain detection. Biomaterials. 2014 Jan;35(1):304-15.

2013 publications

1. Cheng TC, Chuang KH, Chen M, Wang HE, Tzou SC, Su YC, Chuang CH, Kao CH, Chen BM, Chang LS, Roffler SR, Cheng TL*. Sensitivity of PEGylated interferon detection by anti-polyethylene glycol (PEG) antibodies depends on PEG length. Bioconjug Chem. 2013 Aug 5. [Epub ahead of print]
2. Liu WH, Chen YJ, Cheng TL, Lin SR, Chang LS. Cross talk between p38MAPK and ERK is mediated through MAPK-mediated protein phosphatase 2A catalytic subunit α and MAPK phosphatase-1 expression in human leukemia U937 cells. Cell Signal. 2013 Sep;25(9):1845-51
3. Chen SH, Kuo YT, Cheng TL, Chen CY, Chiu YY, Lai JJ, Chang CC, Jaw TS, Wang YM, Liu GC. In vivo magnetic resonance imaging of mice liver tumors using a new gadolinium-based contrast agent. Kaohsiung J Med Sci. 2013 May;29(5):246-53.
4. Cheng CM, Chen FM, Lu YL, Tzou SC, Wang JY, Kao CH, Liao KW, Cheng TC, Chuang CH, Chen BM, Roffler S, Cheng TL*. Expression of β-glucuronidase on the surface of bacteria enhances activation of glucuronide prodrugs. Cancer Gene Ther. 2013 May;20(5):276-81
5. Wu SC, Lin KL, Wang TP, Tzou SC, Singh G, Chen MH, Cheng TL, Chen CY, Liu GC, Lee TW, Hu SH, Wang YM. Imaging specificity of MR-optical imaging agents following the masking of surface charge by poly(ethylene glycol). Biomaterials. 2013 May;34(16):4118-27.
6. Lu CY, Tsai HL, Uen YH, Hu HM, Chen CW, Cheng TL, Lin SR, Wang JY. Circulating tumor cells as a surrogate marker for determining clinical outcome to mFOLFOX chemotherapy in patients with stage III colon cancer. Br J Cancer. 2013 Mar 5;108(4):791-7.

2012 publications

1. Chen SH, Kuo YT, Singh G, Cheng TL, Su YZ, Wang TP, Chiu YY, Lai JJ, Chang CC, Jaw TS, Tzou SC, Liu GC, Wang YM. Development of a Gd(III)-Based Receptor-Induced Magnetization Enhancement (RIME) Contrast Agent for β-Glucuronidase Activity Profiling. Inorg Chem. 2012 Nov 1. [Epub ahead of print] 2011 IF=4.601, (4/44=9%) in CHEMISTRY, INORGANIC & NUCLEAR
2. Lin YJ, Liu YS, Yeh HH, Cheng TL, Wang LF. Self-assembled poly(ε-caprolactone)-g-chondroitin sulfate copolymers as an intracellular doxorubicin delivery carrier against lung cancer cells. Int J Nanomedicine. 2012;7:4169-83. 2011 IF=3.130, (70/261=26.8%) in PHARMACOLOGY & PHARMACY
3. Chen CH, Liu YK, Lin YL, Chuang HY, Hsu WT, Chiu YH, Cheng TL, Liao KW.A rapid and convenient method to enhance transgenic expression in target cells. Prep Biochem Biotechnol. 2012;42(5):448-61. 2011 IF=0.466, (279/290=96.2%) in BIOCHEMISTRY & MOLECULAR BIOLOGY
4. Kao CH, Cheng CM, Chuang KH, Chuang CH, Tzou SC, Cheng TC, Hsieh YC, Liao KW, Wang YM, Chang LS, Roffler SR, Chen FM, Cheng TL*. A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis. Anal Biochem. 2012 Dec 1;431(1):1-3. 2011 IF=2.996 (19/73=26%) in CHEMISTRY, ANALYTICAL
5. Chen CP, Hsieh YT, Prijovich ZM, Chuang HY, Chen KC, Lu WC, Tseng Q, Leu YL, Cheng TL, Roffler SR. ECSTASY, an adjustable membrane-tethered/soluble protein expression system for the directed evolution of mammalian proteins. Protein Eng Des Sel. 2012 25(7):367-75. 2011 IF=2.937, (47/160=29.3%) in Biotechnology & Applied Microbiology
6. Cheng TL, Chuang KH, Chen BM, Roffler SR. Analytical Measurement of PEGylated Molecules. Bioconjug Chem. 2012 Feb 2. 23, 881−899. 2011 IF: 4.930 (8/56=14.2%) CHEMISTRY, ORGANIC
7. Ta-Chun Cheng, Steve R. Roffler, Shey-Cherng Tzou, Kuo-Hsiang Chuang, Yu-Cheng Su, Chih-Hung Chuang, Chien-Han Kao, Chien-Shu Chen,I-Hong Harn, Kuan-Yi Liu, Tian-Lu Cheng* and Yu-Ling Leu*. An Activity-Based Infrared Glucuronide Trapping Probe for Imaging b-Glucuronidase Expression in Deep Tissues. J. Am. Chem. Soc. 2012, 134, 3103−3110. 2011 IF=9.907, (11/154=7.1%) in CHEMISTRY, MULTIDISCIPLINARY
8. CH Chuang, KH Chuang, HE Wang, SR Roffler, SC Tzou, TC Cheng, CH Kao, SY Wu, WL Tseng, J Shiea, JM Wang* and TL Cheng*. In vivo PET Imaging of protease activity by hydrophilic-to-hydrophobic conversion of a protease substrate. Clin Cancer Res. 2012 Jan 1;18(1):238-247. 2011 IF=7.742, (14/196=7.1%) in ONCOLOGY
9. Chen CH, Lin YL, Liu YK, He PJ, Lin CM, Chiu YH, Wu CJ, Cheng TL, Liu SJ, Liao KW. Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch. Int J Nanomedicine. 2012;7:607-21. Epub 2012 Feb 3.
10. Huang MY, Wang HM, Tok TS, Chang HJ, Chang MS, Cheng TL, Wang JY, Lin SR. EVI2B, ATP2A2, S100B, TM4SF3, and OLFM4 As Potential Prognostic Markers for Postoperative Taiwanese Colorectal Cancer Patients. DNA Cell Biol. 2012 Apr;31(4):625-35.

2011 publications

1. Kasala D, Lin TS, Chen CY, Liu GC, Kao CL, Cheng TL, Wang YM. [Gd(Try-TTDA)(H2O)]2-: a new MRI contrast agent for copper ion sensing. Dalton Trans. 2011 May 14;40(18):5018-25.
2. Tseng FJ, Liu YK, Chung YS, Lin YL, Chen CH, Wang WY, Chen YC, Tsai NM, Cheng TL, Pan RY, Hu TM, Lee RP, Liao KW A fusion protein composed of receptor binding domain of vascular endothelial growth factor-A and constant region fragment of antibody: angiogenesis antagonistic activity. Cytotechnology. 2011 May;63(3):285-93.
3. Chen KC, Wu SY, Leu YL, Prijovich ZM, Chen BM, Wang HE, Cheng TL, Roffler SR. A humanized immunoenzyme with enhanced activity for glucuronide prodrug activation in the tumor microenvironment. Bioconjug Chem. 2011 May 18;22(5):938-48.
4. Huang PT, Chen KC, Prijovich ZM, Cheng TL, Leu YL, Roffler SR.Enhancement of CPT-11 antitumor activity by adenovirus-mediated expression of β-glucuronidase in tumors. Cancer Gene Ther. 2011 Jun;18(6):381-9.
5. Liu YK, Lin YL, Chen CH, Lin CM, Ma KL, Chou FH, Tsai JS, Lin HY, Chen FR, Cheng TL, Chang CC, Liao KW A unique and potent protein binding nature of liposome containing polyethylenimine and polyethylene glycol: a nondisplaceable property. Biotechnol Bioeng. 2011 Jun;108(6):1318-27

2010 publications

1. Kuo-Hsiang Chuang, Hsin-Ell Wang, Ta-Chun Cheng, Shey-Cherng Tzou, Chiu-Min Cheng, Chien-Han Kao, Chih-Hung Chuang3, Wei-Lung Tseng, Wen-Chun Hung, Ming-Hong Tai, Tien-Kuei Chang, Bing-Mae Chen, Steve R. Roffler and Tian-Lu Cheng*. Development of an Universal Anti-Polyethylene Glycol Reporter Gene for Noninvasive Imaging of PEGylated Probes. Journal of Nuclear Medicine, 2010, 51:933-941
2. 2009IF: 6.424 (1/104 0.96%) RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
3. Kuo-Hsiang Chuang, Shey-Cherng Tzou, Ya-Chen Chang, Chien-Han Kao, Kuang-Wen, Liao, Ta-Chun Cheng, Bo-Jyun Huang, Chang-Jer Wu, Wei-Lung Tseng, Jentaie Shiea, Pei-Yu Chu, Steve Roffler, Tian-Lu Cheng* Measurement of Free Polyethylene Glycol by a Novel Cell-Based Anti-Polyethylene Glycol ELISA. Anal Chem. 2010 Mar 15;82(6):2355-62. 2009IF: 5.214 (3/70 4.29%) CHEMISTRY, ANALYTICAL
4. Kuo-Hsiang Chuang, Hsin-Ell Wang, Fang-Ming Chen, Shey-Cherng Tzou, Chiu-Min Cheng, Ya-Chen Chang, Wei-Lung Tseng, Jentaie Shiea, Shiu-Ru Lin, Jaw-Yuan Wang, Bing-Mae Chen, Steve R. Roffler and Tian-Lu Cheng*. Endocytosis of PEGylated agents enhances cancer imaging and anti-cancer efficacy. Molecular cancer therapeutics, 2010, 9(6);1903-1912 2009IF: 4.953 (27/165 16.36%) ONCOLOGY
5. Tsao DA, Chang HJ, Lin CY, Hsiung SK, Huang SE, Ho SY, Chang MS, Chiu HH, Chen YF, Tian-Lu Cheng*, Shiu-Ru L. Gene expression profiles for predicting the efficacy of the anticancer drug 5-fluorouracil in breast cancer. DNA Cell Biol. 2010 Jun;29(6):285-93. 2009IF: 2.280  (170/283 60.07%) BIOCHEMISTRY & MOLECULAR BIOLOGY
6. Su YC, Chen BM, Chuang KH, Tian-Lu Cheng*, Roffler SR. Sensitive Quantification of PEGylated Compounds by Second-Generation Anti-Poly(ethylene glycol) Monoclonal Antibodies. Bioconjug Chem. 2010 Jul 21;21(7):1264-70. 2009IF: 4.305 (7/57 12.28%) CHEMISTRY, ORGANIC
7. Chung FY, Cheng TL, Chang HJ, Chiu HH, Huang MY, Chang MS, Chen CC, Yang MJ, Wang JY, Lin SR. Differential gene expression profile of MAGE family in taiwanese patients with colorectal cancer. J Surg Oncol. 2010 Aug 1;102(2):148-53. 2009IF: 2.502 (37/166 22.29%) SURGERY
8. Yu CJ, Lin CY, Liu CH, Cheng TL, Tseng WL. Synthesis of poly(diallyldimethylammonium chloride)-coated Fe(3)O(4) nanoparticles for colorimetric sensing of glucose and selective extraction of thiol. Biosens Bioelectron. 2010 Oct 15;26(2):913-7.. 2009IF: 5.429 (2/70 2.86%) CHEMISTRY, ANALYTICAL
9. Yang Yu Hsiang, Yung-Hsin Wang, Jaw-Yuan Wang, Fang-Ming Chen, Wei-Lung Tseng, Che-Hsin Lin, Ming-Chia Hsieh, Ming-Yii Huang, Shiu-Ru Lin and Tian-Lu Cheng*. Simultaneous detection of multiple single nucleotide polymorphisms by a simple SNP chip. Genet Test Mol Biomarkers. 2010 Oct;14(5):653-9. 2009 IF1.17 (119/14681.5) GENETICS & HEREDITY
10. Li YC, Chen BM, Wu PC, Cheng TL, Kao LS, Tao MH, Lieber A, Roffler SR. Cutting Edge: mechanical forces acting on T cells immobilized via the TCR complex can trigger TCR signaling.. J Immunol. 2010 Jun 1;184 (11):5959-63.
11. 2009IF: 5.646 (18/128 14.06%) IMMUNOLOGY
12. Lin CS, He PJ, Tsai NM, Li CH, Yang SC, Hsu WT, Wu MS, Wu CJ, Cheng TL, Liao KW. A potential role for Helicobacter pylori heat shock protein 60 in gastric tumorigenesis. Biochem Biophys Res Commun. 2010 Feb 5;392(2):183-9. 2009IF: 2.548 (146/283 51.59%) BIOCHEMISTRY & MOLECULAR BIOLOGY
13. Lin LF, Chuang CH, Li CF, Liao CC, Cheng CP, Cheng TL, Shen MR, Tseng JT, Chang WC, Lee WH, Wang JM. ZBRK1 acts as a metastatic suppressor by directly regulating MMP9 in cervical cancer. Cancer Res. 2010 Jan 1;70(1):192-201. 2009IF: 7.543  (13/16 7.88%) ONCOLOGY

2009 publications

1. Shey-Cherng Tzou, Steve Roffler, Kuo-Hsiang Chuang, Hsin-Pei Yeh, Chien-Han Kao, Yu-Cheng Su, Chiu-Min Cheng, Wei-Lung Tseng, Jentaie Shiea, Kai-Wen Cheng, Bing-Mae Chen, Tian-Lu Cheng*and Hsin-Ell Wang. Micro-PET imaging of b-glucuronidase activity by the hydrophobic conversion of a glucuronide probe. Radiology, 2009, Sep;252(3):754-62
2. 2009IF: 6.341  (2/104 1.92%) RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
3. Chiu-Min Cheng, Pei-Yu Chu, Kuo-Hsiang Chuang, Steve R. Roffler, Chien-Han Kao, Wei-Lung Tseng, Jentaie Shiea, Wen-Da Chang, Yu-Cheng Su, Bing-Mae Chen, Yun-Ming Wang and Tian-Lu Cheng*. Hapten-derivatized nanoparticle targeting and imaging of gene expression by multimodality imaging systems. Cancer gene therapy, 2009 Jan;16(1):83-90. (2009IF: 3.126   (41/150 27.33%) BIOTECHNOLOGY & APPLIED MICROBIOLOGY
4. Chen YM, Cheng TL, Tseng WL. Fluorescence turn-on detection of iodide, iodate and total iodine using fluorescein-5-isothiocyanate-modified gold nanoparticles. Analyst. 2009 Oct;134(10):2106-12. 2009IF: 3.272  (11/70 15.71%) CHEMISTRY, ANALYTICAL
5. Li MD, Tseng WL, Cheng TL. Ultrasensitive detection of indoleamines by combination of nanoparticle-based extraction with capillary electrophoresis/laser-induced native fluorescence J Chromatogr A. 2009 Sep 4;1216(36):6451-8. 2009 IF: 4.101  (4/70 8.5%) CHEMISTRY, ANALYTICAL
6. Yu CJ, Cheng TL, Tseng WL. Effects of Mn2+ on oligonucleotide-gold nanoparticle hybrids for colorimetric sensing of Hg2+: improving colorimetric sensitivity and accelerating color change. Biosens Bioelectron. 2009 Sep 15;25(1):204-10. 2009IF:5.429 (2/70 2.86%)CHEMISTRY, ANALYTICAL
7. Chung FY, Huang MY, Yeh CS, Chang HJ, Cheng TL, Yen LC, Wang JY, Lin SR. GLUT1 gene is a potential hypoxic marker in colorectal cancer patients. BMC Cancer. 2009 Jul 20;9:241. 2009 IF: 2.736 (71/165 43.03%) ONCOLOGY
8. Liu GS, Huang HT, Lin CJ, Shi JY, Liu LF, Tseng RT, Weng WT, Lam HC, Wen ZH, Cheng TL, Hsu KS, Tai MH. Prophylactic Proopiomelanocortin Expression Alleviates Capsaicin-Induced Neurogenic Inflammation in Rat Trachea. Shock. 2009 Dec;32(6):645-50.2008IF: 2.871  (23/166 13.86%) SURGERY
9. Cheng SC, Cheng TL, Chang HC, Shiea J. Using laser-induced acoustic desorption/electrospray ionization mass spectrometry to characterize small organic and large biological compounds in the solid state and in solution under ambient conditions. Anal Chem. 2009 Feb 1;81(3):868-74. 2009IF: 5.214 (3/70 4.29%) CHEMISTRY, ANALYTICAL
10. Li MD, Cheng TL, Tseng WL. Nonionic surfactant-capped gold nanoparticles for selective enrichment of aminothiols prior to CE with UV absorption detection. Electrophoresis. 2009 Jan;30(2):388-95. 2009IF: 3.077 (13/70 18.57%) CHEMISTRY, ANALYTICAL
11. Chen TJ, Cheng TH, Chen CY, Hsu SC, Cheng TL, Liu GC, Wang YM. Targeted Herceptin-dextran iron oxide nanoparticles for noninvasive imaging of HER2/neu receptors using MRI. J Biol Inorg Chem. 2009 Feb;14(2):253-60. 2009IF: 3.415 (8/44 18.189%) CHEMISTRY, INORGANIC & NUCLEAR

2008 Publications

1. Tian-Lu Cheng and Steve Roffler. Membrane-tethered proteins for basic research, imaging and therapy. Medicinal Research Reviews, 2008, Nov;28(6):885-928 2009IF: 8.656  (8/236 3.39%) PHARMACOLOGY & PHARMACY
2. Chiu-Min Cheng, Yu-Ling Lu , Kuo-Hsiang Chuang,Wen-Chun Hung, Jentaie Shiea, Yu-Cheng Su, Chien-Han Kao, Bing-Mae Chen, Steve Roffler and Tian-Lu Cheng* Tumor-targeting prodrug-activating bacteria for cancer therapy. Cancer Gene Ther. 2008, Jun;15(6):393-401. 2009IF: 3.126   (41/150 27.33%) BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3. Cheng CY, Yuan CH, Cheng SC, Huang MZ, Chang HC, Cheng TL, Yeh CS, Shiea J. Electrospray-assisted laser desorption/ionization mass spectrometry for continuously monitoring the states of ongoing chemical reactions in organic or aqueous solution under ambient conditions. Anal Chem. 2008 Oct 15;80(20):7699-705. 2009IF: 5.214 (3/70 4.29%) CHEMISTRY, ANALYTICAL
4. Wu HP, Huang CC, Cheng TL, Tseng WL. Sodium hydroxide as pretreatment and fluorosurfactant-capped gold nanoparticles as sensor for the highly selective detection of cysteine. Talanta. 2008 Jul 15;76(2):347-52. 2009IF: 3.290  (9/70 12.86%) CHEMISTRY, ANALYTICAL
5. Uen YH, Lu CY, Tsai HL, Yu FJ, Huang MY, Cheng TL, Lin SR, Wang JY. Persistent Presence of Postoperative Circulating Tumor Cells is a Poor Prognostic Factor for Patients with Stage I-III Colorectal Cancer after Curative Resection. Ann Surg Oncol. 2008, Aug;15(8):2120-8.
6. 2009IF: 4.130  (6/166 3.61%)SURGERY
7. Liu FC, Cheng TL, Shen CC, Tseng WL, Chiang MY. Synthesis of Cysteine-Capped Zn(x)Cd(1)(-)(x)Se alloyed quantum dots emitting in the blue-green spectral range. Langmuir. 2008 Mar 4; 24(5):2162-7. 2009IF:3.898 (25/212 11.79%)MATERIALS SCIENCE, MULTIDISCIPLINARY
8. Chen YM, Yu CJ, Cheng TL, Tseng WL. Colorimetric detection of lysozyme based on electrostatic interaction with human serum albumin-modified gold nanoparticles. Langmuir. 2008 Apr 1; 24(7):3654-60. 2009IF:3.898 (25/212 11.79%)MATERIALS SCIENCE, MULTIDISCIPLINARY
9. Huang MY, Wang YH, Chen FM, Lee SC, Fang WY, Cheng TL, Hou MF, Wang JY, Lin SR. Multiple Genetic Polymorphisms of GSTP1 313AG, MDR1 3435CC, and MTHFR 677CC highly correlated with early relapse of breast cancer patients in Taiwan. Ann Surg Oncol. 2008 Mar;15(3):872-80. 2009IF: 4.130  (6/166 3.61%)SURGERY
10. Huang MY, Fang WY, Lee SC, Cheng TL, Wang JY, Lin SR. ERCC2 2251A>C genetic polymorphism was highly correlated with early relapse in high-risk stage II and stage III colorectal cancer patients: a preliminary study. BMC Cancer. 2008 Feb 12;8:50. 2009IF: 2.736 (71/165 43.03%) ONCOLOGY

榮譽

獲獎：

　　2008 Outstanding Research Award (RPI award), Kaohsiung Medical University (2008高醫研究傑出教師獎)

　　2008 Outstanding Industrial Collaboration Award, Kaohsiung Medical University (2008高醫產學傑出績優獎)

　　2008 annual excellent technique transfer award, Kaohsiung Medical University (2008高醫傑出技轉獎)

　　2009 Outstanding Industrial Collaboration Award, Kaohsiung Medical University (2009產學合作--傑出貢獻獎)

　　2009 annual excellent technique transfer award, Kaohsiung Medical University (2009技術移轉--傑出貢獻獎)

　　2009 Outstanding Alumni Award of Excellent Paper, Kaohsiung Medical University (2009高醫校友優秀教師論文獎)

　　2009 annual excellent patent award,Kaohsiung Medical University (2009專利獲證--傑出貢獻獎)

　　2009 annual excellent research project funding,Kaohsiung Medical University (2009研究傑出教師—研究計畫經費最佳獎)

　　2009 Outstanding Research Award (IF>5), Kaohsiung Medical University (2009研究傑出教師—傑出論文獎)

　　2009 Outstanding Research Award (RPI award), Kaohsiung Medical University. (2009研究傑出教師--最佳研究貢獻獎)

　　2010 Outstanding Alumni Award of Excellent Paper, Kaohsiung Medical University (2010高醫校友優秀教師論文獎)

　　2010 annual excellent research project funding, Kaohsiung Medical University (2010研究傑出教師—研究計畫經費最佳獎)

　　2010 Outstanding Research Award (IF>5), Kaohsiung Medical University (2010研究傑出教師—傑出論文獎)

　　2010 Outstanding Research Award, Kaohsiung Medical University. (2010研究傑出教師--最佳研究貢獻獎)

　　2010 annual excellent technique transfer award, Kaohsiung Medical University (2010技術移轉績優)

　　2011 Outstanding Alumni Award of Excellent Paper, Kaohsiung Medical University(2011高醫校友優秀教師論文獎)

　　2011 annual excellent research project funding, Kaohsiung Medical University (2011研究績優教師—研究計畫經費績優獎)

　　2011 annual excellent technique transfer award, Kaohsiung Medical University (2011技術移轉傑出貢獻獎)

　　2012 Outstanding Alumni Award of Excellent Paper, Kaohsiung Medical University(2012高醫校友優秀教師論文獎)

　　2012 Outstanding Research Award (IF>5), Kaohsiung Medical University (2012研究傑出教師—傑出論文獎)

　　2012 annual excellent research project funding, Kaohsiung Medical University (2012研究績優教師—研究計畫經費績優獎)

　　2012 annual excellent patent award,Kaohsiung Medical University (2012專利獲證--傑出貢獻獎)

　　2012 annual excellent technique transfer award, Kaohsiung Medical University (2012技術移轉 卓越獎)

　　2012 Outstanding Industrial Collaboration Award, Kaohsiung Medical University (2012高醫產學合作 卓越獎)

創業競賽：

　　2008第6屆「國家新創獎」學生組第一名"獎金十萬元"
　　技術名稱：聚乙二醇修飾分子之創新定量技術研發
　　隊員：莊國祥．鄭達駿．章雅純．盧賜蓉．陳挺宇
　　指導老師：鄭添祿教授

　　2013第10屆「國家新創獎」學生組第二名"獎金十萬元"
　　技術名稱：一種可屏蔽抗體或受器活性的閉鎖器
　　隊員：呂韻綺、莊智弘、陳怡如、何昇穎、林文瑋、黃柏誠
　　指導老師：鄭添祿教授

　　2013第10屆「國家新創獎」學生組第三名"獎金五萬元"
　　技術名稱：PEG分子偵測晶球
　　隊員：謝元欽、高千涵、鄭以安、江怡宣
　　指導老師：鄭添祿教授

　　連續7&8屆榮獲戰國策全國校園創業競賽~創業組第一名(第8屆同時榮獲創業組第三名)

　　2012第7屆戰國策全國校園創業競賽(245隊參加)~創業組第一名~"獎金五萬元"
　　題目: 開發自動呈色且分子量規則之重組蛋白質標記
　　創 作 人：高千涵、林文瑋、胡翠文、蔡亞倫

　　2013第8屆戰國策全國校園創業競賽(301隊參加)~創業組第一名~"獎金五萬元"
　　題目: 抗體人類化技術平台 創作人：謝元欽、莊智弘、林文偉、曾明洋

　　2013第8屆戰國策全國校園創業競賽(301隊參加)~創業組第三名"獎金一萬元"
　　題目: 檢測PEG drugs動力學之超簡便PEG微米晶球 創作人：高千涵、謝元欽、胡翠文、鄭以安、蔡亞倫、蔡佳蓉、何愷文

　　連續兩屆(第1&2屆)榮獲高醫大「創業競賽」學生組第一、二名

　　2012高醫大「第一屆創業競賽」學生組第一名"獎金五萬元"
　　題目：以新奇抗聚乙二醇捕捉器定量任何聚乙二醇及其修飾分子
　　創作人：謝元欽、江怡宣、曾明洋

　　2012高醫大「第一屆創業競賽」學生組第二名"獎金三萬元"
　　題目：開發自動呈色且分子量規則之重組蛋白質標記
　　創作人：林文瑋、胡翠文、蔡亞倫、高千涵

　　2013高醫大「第二屆創業競賽」學生組第一名"獎金五萬元
　　題目: 抗體鎖
　　創作人：莊智弘、呂韻琦、陳怡如

　　2013高醫大「第二屆創業競賽」學生組第二名 "獎金三萬元"
　　題目: 抗體人類化平台:抗體腦
　　創 作 人：謝元欽、林文偉、曾明洋

　　2013南科創新創意創業競賽第一名"獎金10萬元"(高醫&中山大學)
　　題目：抗體腦
　　創作人：謝元欽、林文偉、莊智弘

　　2013入選國科會創新到創業競賽計畫第二階段"獎金十萬元+籌辦費二十萬";第三階段獎金二十萬元+籌辦費四十萬")
　　題目：微米定量晶球:可定量任何聚乙二醇及其修飾分子之微米晶球
　　參賽人員：鄭添祿教授、莊國祥助理教授、謝元欽、江怡宣

　　2013入圍國家發明獎決審 (I386645)
　　題目：可定量任何聚乙二醇 (PEG)分子與其修飾物之抗聚乙二醇表現細胞
　　發明人：鄭添祿、Steve Roffler、莊國祥、盧賜蓉

專利

專利（A.發明專利 B.新型專利）

　　類別/專利名稱/國別/專利號碼or申請號碼/發明人/專利權人/專利期限/經費補助單位及其計畫編號

1. A/Monoclonal antibody for analysis and clearance of polyethylene glycol and polyethylene glycol-modified molecular(http://www.uspto.gov/patft/index.html) /USA /US6596849 B1/ SR Roffler, TL Cheng and PY Wu. /中研院/ 2003~ /NSC89-2320-B001-017
2. A/Monoclonal antibody for analysis and clearance of polyethylene glycol and polyethylene glycol-modifiedmolecular.(http://www.uspto.gov/patft/index.html).These patents have different claims./USA/US6,617,118/SR Roffler, TL Cheng and PY Wu./Academia Sinica/2003~/NSC89-2320-B001-017
3. A/Monoclonal antibody for analysis and clearance of polyethylene glycol and polyethylene glycol-modifiedmolecular. (http://www.uspto.gov/patft/index.html).These patents have different claims./USA /US7,320,791/ SR Roffler, TL Cheng and PY Wu./Academia Sinica /2008~/NSC89-2320-B001-017
4. A/Method for a biochip detecting limited cells/ ROC/ 專利證號 I287581/ Shiu-Ru. Lin,TL Cheng, Y.-F. Chen, C.-H. Wu/KaohsiungMedicalUniversity/2007~/NSC-91-2324-B-037-324
5. A/US Patent: Title: MEMBRANE BOUND REPORTER GENE SYSTEM Pub.No.: US2008/0176225A1/USA/US2008/0176225A1/TL Cheng, SR Roffler, Yu-Cheng Su /KaohsiungMedicalUniversity & Academia Sinica/Jul.24.2008/NSC92-3112-B-037-001
6. A/大環四胺基單醯胺基三羧酸基雙氟甲苯基醣類化合物作為配位子之具生物活性順磁性金屬錯合物/台灣/專利證號 I314456/Yun-Ming WM Wang張騰耀 TL Cheng 劉金昌/KaohsiungMedicalUniversity(2009/09/00~2026/08/00)
7. A/重組的核苷酸序列、包含其之細胞或載體、所編碼出之單鏈抗聚乙二醇膜抗體與包含其之細胞的使用方法/台灣/台灣專利號 : I390040/TL Cheng, SR Roffler, Kuo-Hsiang Chuang KaohsiungMedicalUniversity(2013/3/21~2029/9/16)/NSC95-3112-B-037-001
8. A/可自動呈色且分子量規則之14 tags重組蛋白分子量標記/USA and台灣 97004-T台灣專利申請;97004-A美國專利申請/TL Cheng and Chiu-Min Cheng/ KaohsiungMedicalUniversity/2010~/98-2622-B-037-001-CC1
9. A/可定量任何聚乙二醇分子與其修飾物之抗聚乙二醇表現細胞/USA and台灣/台灣專利號 : I386645;98017-A美國專利申請中(第二次答辯)/TL Cheng, SR Roffler, Kuo-Hsiang Chuang,Ssu-Jung Lu KaohsiungMedicalUniversity(2013/2/21~2030/7/18)/NSC98-3112-B-037-001
10. A/複合式多標的癌細胞冷光偵測方法/台灣/專利證號I388832/林綉茹, 鄭添祿/KaohsiungMedicalUniversity/2013/03/11~2026/08/23
11. A/HINGE DOMAIN AS LOCKER FOR INACTIVATION OF PROTEIN DRUG/美國臨時案(61827763)/鄭添祿、莊智弘、柯秀芬、呂韻琦/KaohsiungMedicalUniversity/NSC102-2325-B-037-001-
12. A/Non-covalent modification of PEGylated molecules by Anti-PEG bispecific antibodies for targeted therapy/美國臨時案(P2776-US)/鄭添祿、羅傅倫、高千涵、陳炳梅 KaohsiungMedicalUniversity/NSC102-2325-B-037-001-

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